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The effect of the PLA2 inhibitors MAFP (A) and R- and S-BEL (B) on the orexin-A-stimulated AA release in CHO-hOX1 cells. The cells were preincubated for 30 min with the inhibitor, changed to fresh NaBM containing the inhibitor and immediately stimulated with orexin-A. The responses are normalized so that each control response (basal, 1 nM orexin-A, 100 nM orexin-A) amounts to 100% (see Data analysis); ns (not significant), P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.
