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The effect of PKC and ERK activation on AA release in CHO-hOX1 cells. (A) Cells were preincubated for 30 min with the MEK inhibitors U0126 and 98059 as indicated, changed to fresh NaBM containing the inhibitor and immediately stimulated with orexin-A. Data are normalized as explained in Data analysis and Figure 3. (B) Cells were stimulated with 1 mM TPA for 7 min in NaBM. ns (not significant), P > 0.05; **P < 0.01; ***P < 0.001.
