IL-8 production and % migration of neutrophils to oxidative stress using the Transwell system. Linoleic acid (LA) did not stimulate IL-8 production or transendothelial migration of neutrophils as compared to medium control, however, an oxidizing solution containing LA (OxLA) greatly stimulated IL-8 and neutrophil migration. Pretreatment of cells with VE and VC or co-incubation with an IL-8 neutralizing antibody inhibited IL-8 production and neutrophil migration induced by OxLA. b, significantly higher than medium control and LA, P < 0.01; c, significantly lower than OxLA, P < 0.01. Data represent mean ± SE, n = 7 independent experiments with treatments run in duplicate.