Table 1.
Permeant | Protocol | Delivery and enhancing mechanism |
Purpose |
---|---|---|---|
Dextran | 2 mA anodal or cathodal iontophoresis for 10 s |
Electroosmosis and electro- permeabilization |
Determine the effect of electroosmosis |
Dextran | 0.5 mA anodal iontophoresis for 1 min |
Electroosmosis and electro- permeabilization |
Determine the effect of electric current |
Dextran, GAPDH siRNA |
Passive delivery for 1 min | Diffusion, effect of the solution, device physical effect |
Passive control |
GAPDH siRNA | 0.5 mA anodal or cathodal iontophoresis for 1 min |
Electrophoresis, electroosmosis, and electro-permeabilization |
Determine the effect of electrophoresis; examine the delivery over 24 hr |
GAPDH siRNA | 20 V (8 pulses, 50 ms length, 1 s interval) anodal or cathodal electroporation |
Electroporation and diffusion | Examine the delivery over 24 hr; compare electroporation with iontophoresis |
GAPDH siRNA | Combined 0.5 mA cathodal iontophoresis and 20 V anodal (or cathodal) electroporation |
Electrophoresis, electroosmosis, electro-permeabilization, and diffusion |
Examine the delivery over 24 hr |
n ≥ 3 replicates in each experimental condition.