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Binding detection of wt or mutant IL-31 on the GO-G-UVM cell line by flow cytometry analysis. 700 ng/ml of wild type (W.T.) or mutant proteins were incubated for 1 h with the GO-G-UVM cell line. Indirect detection of the binding was then performed using an anti-V5 mAb followed by a phycoerythrin-conjugated anti-mouse antibody.
