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Analysis of STAT-3, MAPK, and Akt phosphorylation induced by wt or mutant IL-31. The GO-G-UVM cell line was incubated for 10 min in the presence of wt (W.T.) or mutant IL-31 forms (10 ng/ml for STAT-3 analysis and 50 ng/ml for MAPK and Akt analysis), before being lysed and subjected to Western blotting using anti-phospho-STAT-3 (STAT3-P), anti-STAT-3 (STAT3), anti-phospho-MAPK (MAPK-P), anti-MAPK (MAPK), anti-phospho-Akt (Akt-P), and anti-Akt (Akt) antibodies.
