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. 2009 Nov 19;285(5):3103–3113. doi: 10.1074/jbc.M109.053249

FIGURE 4.

FIGURE 4.

Cysteines of TMD 4 and 10 are required for full charge movement. A, NLC curves constructed from mean fit parameters show that C192S/C196S and C415S prestin have reduced area under the curve, indicating reduced charge movement compared with WT prestin. V½ and z for these mutants are consistent with WT measures (see Figs. 3 and 5). Mutants with normal charge movement capabilities show similar area under the curve compared with WT prestin, and C679S prestin is shown as an example. B, most serine substitutions provided normal charge movement of ∼15 fC/pF; however, C192S/C196S and C415S prestin exhibited significantly reduced levels compared with WT controls (p < 0.01) as follows: WT, 18.27 ± 1.89 fC/pF (n = 21); C52S, 15.46 ± 2.15 fC/pF (n = 11); C124S, 11.46 ± 2.28 fC/pF (n = 8); C192S/C196S, 7.37 ± 0.81 fC/pF (n = 7); C260S, 16.54 ± 2.75 fC/pF (n = 12); C381S, 15.79 ± 2.14 fC/pF (n = 7); C395S, 15.86 ± 2.19 fC/pF (n = 10); C415S, 7.07 ± 1.33 fC/pF (n = 12); C679S, 16.59 ± 2.69 fC/pF (n = 10). All values are mean ± S.E. WT range is highlighted by a gray horizontal bar.