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. 2009 Nov 19;285(5):3103–3113. doi: 10.1074/jbc.M109.053249

FIGURE 5.

FIGURE 5.

Cysteine substitution in TMD 6 alters the mechanics of charge movement. A, NLC curves were constructed from mean fit parameters. Because V½ was consistent with WT prestin (see Fig. 3), C260S prestin NLC was translated and aligned with WT to illustrate the effects of increased valence of charge movement. C260S had significantly higher valence than WT prestin (p < 0.01), which corresponds to a narrower voltage range over which the prestin state transition occurs. Charge density for this mutant did not differ from WT prestin (see Fig. 4). B, z for all groups is as follows: WT, 0.67 ± 0.01 (n = 21); C52S, 0.76 ± 0.02 (n = 11); C124S, 0.73 ± 0.03 (n = 8); C192S/C196S, 0.70 ± 0.02 (n = 7); C260S, 0.80 ± 0.01 (n = 12); C381S, 0.70 ± 0.03 (n = 7); C395S, 0.67 ± 0.02 (n = 10); C415S, 0.69 ± 0.02 (n = 12); C679S, 0.72 ± 0.02 (n = 10). All values are dimensionless (mean ± S.E.). WT range is highlighted by a gray horizontal bar.