FIGURE 1.

CHOphox cells respond to PMA stimulation. CHO cells stably expressing the NADPH oxidase components gp91^phox, p22^phox, p47^phox, and p67^phox (CHOphox) were incubated at the indicated cell concentrations and stimulated with buffer or 100 nm PMA in the presence of 100 μl Diogenes. A, RLU were recorded over 60 min for unstimulated (None; open symbols) and stimulated (PMA; closed symbols) CHOphox cells at various concentrations. Data are from one experiment and are representative of three performed. B, summarized chemiluminescence rate data from three experiments (mean ± S.E. (error bars)) of RLU produced from 20 to 40 min are shown. C, whole cell lysates were subjected to SDS-PAGE and Western blot analysis as described under “Experimental Procedures.” Blots were probed for gp91^phox, p22^phox, p47^phox, and p67^phox and are representative of three separate experiments.