FIGURE 3.

PMA-stimulated NADPH oxidase activity by CHO 91/47/67 cells expressing p22^phox point mutations. CHO 91/47/67 cells expressing either no p22^phox (Mock), wild type p22^phox (wtp22), Pro-156 to Gln p22^phox (P156Q), Thr-132 to Ala p22^phox (T132A), or Thr-147 to Ala p22^phox (T147A) were stimulated at 2 × 10⁶ cells/ml in the presence of 100 μl of Diogenes with either buffer (open bars) or 100 nm PMA (filled bars) for 1–2 h. Data represent four separate transfections and are expressed as mean ± S.E. (error bars) of the RLU/min measured from each p22^phox condition. NS, not significant; *, p < 0.05. Western blot analysis for expression of p22^phox and p47^phox was performed as described under “Experimental Procedures,” and the results from a representative experiment are shown.