FIGURE 2.

The P2Y₂R translocation in membrane rafts is ligand-dependent. To examine whether the P2Y₂R association with membrane raft depends on the occupation of the P2Y₂R, HCA were perfused for 4 min with either 10 μm ATP, 1 μm UTP, or 1 μm UDP. A, representative immunoblots showing that all of these nucleotides induced the association of the P2Y₂R receptor to membrane rafts. Neither 1 μm uridine nor 100 nm ADP or 10 nm MRS 2365 evoked the receptor displacement into raft domains, as shown in representative immunoblots shown in B. Quantification of the immunoblots illustrates the fraction of the P2Y₂R associated with membrane rafts evoked by these nucleotide and their analogs (C). Columns, mean values; bars, S.E. *, p < 0.05 as compared with the controls (n = 3–4 per assay).