FIGURE 4.

UTP induced a rapid association of the P2Y₂R with membrane rafts. Kinetics of the P2Y₂R association with membrane rafts. HCA segments were perfused ex vivo with 1 μm UTP for 1, 2, and 4 min (n = 3 each). A shows representative immunoblots from sucrose density gradients. UTP elicited a rapid enrichment of the P2Y₂R into raft fractions after a 1-min perfusion with the ligand compared with a control protocol perfused without the agonist addition. Quantification of these experiments are depicted in B. Columns, mean values; bars, S.E. (n = 4). *, p < 0.05 when comparing control versus UTP-perfused tissues.