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The pH optimum for matriptase activation in the intact cell activation assay. 184 A1N4 cells were exposed to citric acid-phosphate-based (upper panels) or phosphate-based (lower panels) buffers with the pH indicated, at room temperature for 20 min. Cell lysates were then prepared and analyzed by immunoblotting for total matriptase using mAb M32, for activated matriptase using mAb M69, and for HAI-1 using mAb M19.
