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NHE inhibition induces matriptase activation and shedding. 184 A1N4 cells were incubated in basal media (lane 1) or treated with 0.1% Me2SO (lane 2), 50 μm benzamil (lane 3), 50 μm amiloride (lane 4), 50 μm dimethyl amiloride (DMA) (lane 5), 50 μm EIPA (lane 6), or 50 μm MIBA (lane 7) for 2 h. Cell lysates and culture medium were collected and analyzed for total matriptase, activated matriptase, and HAI-1, as indicated.
