TABLE 4.

Rates of anaerobic flavin reduction of nNOS_red proteins by excess NADPH at 600 nm

Reductions were carried out in the stopped-flow instrument at 10 °C with a 10-fold excess of NADPH while monitoring the absorbance changes at 600 nm as described under “Experimental Procedures.” All rates are the result of fitting absorbance changes to a single exponential function.

Enzyme	Conditions	Increase	Decrease
		s−1	s−1
Wild type	Without CaM	56 ± 1.4	2.3 ± 0.2
	With CaM	160 ± 6	8 ± 1
K842E	Without CaM	253 ± 6	42 ± 3
	With CaM	290 ± 7.5	54 ± 5