FIGURE 2.
Recognition of chemically cross-linked oligomeric Aβ species by antibodies targeting residues 3–7 of Aβ1–40. Chemically cross-linked Aβ1–40, prepared as described under “Experimental Procedures,” was immunoprecipitated using monoclonal antibodies indicated, and the immunoprecipitates were visualized on Western blots following SDS-PAGE on 18% Tricine gels using antibody 3D6 recognizing residues 1–5 of Aβ. A positive (+) notation indicates potency for reversal of behavioral deficit following treatment with antibody in CFC assay (+++ denotes 1 MPK, and + denotes 30 MPK), and a negative (−) notation indicates a lack of effect following treatment with treatment with the antibody in the CFC assay. Activity in CFC is seen to correlate inversely with recognition of monomeric Aβ species by the respective monoclonal antibody.