FIGURE 3.
FVIIa·TF amidolytic activity. Varying concentrations of rTF1–243 (●) and rTF1–243“D” (○) in a, rTF1–263G (■) and rTF1–263D (□) in b, and pTF (♦) and pTFD (◇) in c were incubated with FVIIa (0.5 nm) in HBS, 0.1% polyethylene glycol, 2 mm CaCl2, pH 7.4, for 10 min. Fluorogenic substrate was added (50 μm), and the rate of substrate hydrolysis was recorded. The inset in each graph represents the calculated 1:1 stoichiometry of the TF·FVIIa complex. Each point is a mean of two experiments ± 1 S.D.