TABLE 2.

Binding of TF to FVIIa in a fluorogenic assay

TF species	Kd(app)	Vmaxa	Stoichiometry (TF/FVIIa)
	nm	pm/s
rTF1–243	0.41 ± 0.40	59.7 ± 1.2	1.1:1.0
rTF1–243“D”	0.47 ± 0.03	61.2 ± 1.0	0.9:1.0
rTF1–263G	0.31 ± 0.02	75.8 ± 1.6	0.9:1.0
rTF1–263D	0.35 ± 0.02	78.3 ± 1.4	1.0:1.0
pTFG	0.92 ± 0.15	173.1 ± 8.4	0.9:1.0
pTFD	0.69 ± 0.09	172.8 ± 10.0	1.0:1.0

^a Maximum rate of substrate fluorogenic hydrolysis at 0.5 nm FVIIa and saturating TF.