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. 2009 Nov 23;285(5):3235–3244. doi: 10.1074/jbc.M109.060616

FIGURE 2.

FIGURE 2.

The editing property of AaLeuRS, EcLeuRS, and mutated derivatives. A, shown is a TLC-based AMP formation assay of AaLeuRS or AaLeuRS-T273R in the presence of 5 μm AatRNALeu and 15 mm Nva. B, shown is a graphical representation of AMP formation. kobs of AMP formation was calculated from the slope and reported in Table 1. C, shown is hydrolysis of 1 μm Ile-AatRNALeu by 20 nm AaLeuRS or AaLeuRS-T273R. D, shown is AMP formation assay of EcLeuRS and EcLeuRS -T252R in the presence of 5 μm EctRNALeu and 15 mm Nva. E, shown is a graphical representation of the AMP formation. F, shown is hydrolysis of 1 μm Ile-EctRNALeu by 20 nm EcLeuRS or its EcLeuRS-T252R. ●, AaLeuRS-T273R; ○, AaLeuRS; ■, EcLeuRS-T252R; □, EcLeuRS.