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Isoleucylation of tRNALeu by AaLeuRS, EcLeuRS, and their editing-defective mutants. A, shown is isoleucylation of 20 μm AatRNALeu by 3 μm AaLeuRS (●), AaLeuRS-T273R (○), or AaLeuRS-Y358D (▼) and isoleucylation of 20 μm AatRNALeu by 3 μm AaLeuRS or AaLeuRS-T273R, which is also shown in inset a. B, shown is isoleucylation of 20 μm EctRNALeu with isoleucine by 0.5 μm EcLeuRS (●), EcLeuRS-T252R (○), or EcLeuRS-Y330D (▼).
