TABLE 1.
Observed rate constants of AaLeuRS and its mutants in AMP synthesis at 60 °C
Rates were determined using the AMP formation in the TLC assay described under “Experimental Procedures.” All rates represent the average of three trials with the S.D. indicated.
| LeuRS | tRNA | AMP formation kobs |
|---|---|---|
| s−1 | ||
| Wild type | − | (8.6 ± 1.4) × 10−2 |
| Wild type | + | 1.43 ± 0.25 |
| T273R | − | (9.1 ± 1.4) × 10−2 |
| T273R | + | 1.01 ± 0.17 |
| Y358D | − | (8.8 ± 1.4) × 10−2 |
| Y358D | + | (9.5 ± 1.6) × 10−2 |
| Y358E | − | (9.2 ± 1.4) × 10−2 |
| Y358E | + | (1.7 ± 1.6) × 10−1 |