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. 2009 Nov 23;285(5):3168–3180. doi: 10.1074/jbc.M109.056846

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Effect of antibodies Ab51, AbGSCA206, Ab75, and AbGSCB367 on the generation and electron transfer activity mediated by NQR. Isolated NQR (0.91 mg/ml) was incubated with preimmune IgG or Ab51 or AbGSCA206 or Ab75 or AbGSCB367 (0.36 mg/ml) at 4 °C for 12 h. The generation activity (black bars) was analyzed by EPR spin trapping with DEPMPO in the absence of Q₁, and the electron transfer activity (dashed bars) of Q₁-stimulated NADH oxidation was assayed at room temperature. The spin quantitation for each spectrum was obtained by double integration of the simulation spectrum. The instrumental settings are described under “Experimental Procedures.”

Inline graphic — Effect of antibodies Ab51, AbGSCA206, Ab75, and AbGSCB367 on the generation and electron transfer activity mediated by NQR. Isolated NQR (0.91 mg/ml) was incubated with preimmune IgG or Ab51 or AbGSCA206 or Ab75 or AbGSCB367 (0.36 mg/ml) at 4 °C for 12 h. The generation activity (black bars) was analyzed by EPR spin trapping with DEPMPO in the absence of Q₁, and the electron transfer activity (dashed bars) of Q₁-stimulated NADH oxidation was assayed at room temperature. The spin quantitation for each spectrum was obtained by double integration of the simulation spectrum. The instrumental settings are described under “Experimental Procedures.”