TABLE 1.
Results from FCS measurements
Values are given as average ± S.E. “Starved” indicates measurements before serum addition, and “+serum” data were acquired in the cytoplasm from 1 to 10 min after serum addition and in the nucleus from 10 to 15 min. F/N is fluorescence intensity per mobile object (see under “Experimental Procedures”).
| F/N | D | N | |
|---|---|---|---|
| counts/object/s | μm2/s | ||
| GFP cytoplasm | 319 ± 10 | 17.5 ± 1.3 | 7 |
| GFP nucleus | 308 ± 8 | 16.1 ± 0.7 | 18 |
| WT cytoplasm starved | 316 ± 8 | 7.7 ± 0.4 | 31 |
| WT cytoplasm +serum | 308 ± 5 | 6.6 ± 0.3 | 49 |
| WT nucleus starved | 283 ± 14 | 7.1 ± 0.8 | 13 |
| WT nucleus +serum | 299 ± 11 | 6.8 ± 0.6 | 19 |
| Δ4 cytoplasm starved | 346 ± 8 | 6.4 ± 0.4 | 16 |
| Δ4 cytoplasm +serum | 309 ± 9 | 6.7 ± 0.4 | 9 |
| Δ4 nucleus starved | 296 ± 13 | 5.2 ± 0.4 | 17 |
| Δ4 nucleus +serum | 278 ± 10 | 5.7 ± 0.4 | 20 |