FIGURE 8.

LAB differentially regulates production of IL-10 and IL-12p40 by macrophages. A, BMMΦ from WT and Lat2^−/− mice were stimulated for the indicated times with LPS, and phospho-Erk was assessed by immunoblotting (IB) whole cell lysates. B, BMMΦ from WT and Lat2^−/− mice were stimulated for 24 h with various concentrations of LPS as indicated. Supernatants were quantified for IL-10, IL-12p40, and TNFα by enzyme-linked immunosorbent assay. Black bars represent WT, and gray bars represent Lat2^−/−. Results are representative of at least three independent experiments with a pool of three mice in each.