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Expression and purification of LysM RLK1. LysM RLK1-yEGFP overexpressed in yeast membrane was solubilized and purified by a nickel column. The samples were separated on a 6% SDS gel and analyzed by in-gel fluorescence (left) and CBB staining (right). Lane 1, crude membrane containing LysM RLK1-yEGFP; lane 2, solubilized membrane proteins by 1% LDAO; lane 3, flow-through of the nickel column; lane 4, eluted proteins from the nickel column. *, LysM RLK1 yEGFP.
