FIGURE 4.

Competition of glycol chitin and glycol chitosan for the binding of LysM RLK1-yEGFP to chitin beads. A, 250 nm LysM RLK1-yEGFP incubated with 400 μg/ml glycol chitin and glycol chitosan and then chitin beads was added to the solution and incubated for 20 min. After the incubation, the beads were directly observed by a fluorescence microscope, and the fluorescence images are shown. B, the amount of proteins bound to chitin beads was analyzed by ImageJ. C, competition of the binding of LysM RLK1-yEGFP to chitin beads by glycol chitin and glycol chitosan was analyzed by the addition of increasing concentrations (400, 40, 4, 0.4, 0.04, and 0.004 μg/ml) of them. The experiments were repeated three times with similar results. The relative percentage binding was calculated by comparison to the fluorescence intensity of beads in the absence of competitors (100% binding).