FIGURE 5.

Competition of chitin oligosaccharides and a chitosan oligosaccharide (GlcN)₆ for the binding of LysM RLK1-yEGFP to chitin beads. A, 250 nm LysM RLK1-yEGFP was incubated with a 250 μm concentration of various chitin oligosaccharides and a chitosan oligosaccharide (GlcN)₆ and subsequently, the chitin beads were added to the solution and incubated for 20 min. After the incubation, the beads were directly observed by a fluorescence microscope. B, the amounts of LysM RLK1-yEGFP bound to chitin beads were analyzed by ImageJ. C, competition of the binding of LysM RLK1 to chitin beads by chitin and chitosan oligosaccharides was analyzed by the addition of increasing concentrations (500, 50, 5, 0.5, and 0.05 μm) of them. The experiments were repeated three times with similar results. The relative percentage binding was calculated in the same way as in Fig. 4.