FIGURE 3.
a, the mutant CASQ2D307H gets ubiquitinated at the same rate as the WT CASQ2. Whole heart homogenates from WT and CASQ2D307H (transgenic) (n = 4) were enriched for ubiquitinated proteins, separated on a 10% SDS gel, and probed for CASQ2 (left) and ubiquitin (right). The experiment was repeated 4 times. b, expression of CASQ2D307H protein does not affect the expression level of ER chaperone proteins GRP94 and GRP78 relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as a standard.