FIGURE 3.

Cadein1 sensitivity of a p53-mutant colon cancer cell line is increased with functional MMR. A, HCT116-Ch3/E6, HCT116-E6, HCT116-Ch3, and HCT116 cells were treated with various concentrations of cadein1 for 24 h. Cell viability was assessed by MTT assay. Three independent experiments were performed at each point, and the mean value was plotted with S.D. B and C, HCT116-Ch3/E6, HCT116-E6, HCT116-Ch3, and HCT116 cells were treated with 4 μm cadein1 for the indicated times. Cell extracts were immunoblotted with anti-PARP (B) and anti-cleaved capapse-3 antibodies (C). Actin (B) and α-tubulin (C) were used as loading controls.