FIGURE 5.
Inhibition of β-catenin/TCF-dependent transcription by ICAT promotes cell death in AT2 cells. A, cells were infected (triplicate wells) with adenoviral constructs at various plaque forming units/cell on Day0 and cultured for 3 days. Culture media was collected, cleared, and assayed for LDH as an indicator of cell death using a Roche Applied Science LDH kit. Cell death in MDCK cells, which have much less of the signaling form of β-catenin than AT2 cells (Western blot (B)), was not affected by ICAT or constitutively active S37A β-catenin. Each bar represents the mean of three independent experiments (i.e. from three different AT2 isolations and infections) ± S.D. C, ICAT-induced cell death can be partially rescued by co-infection with an adenovirus expressing the anti-apoptotic, Bcl-XL. Each bar represents the mean of three independent experiments ± S.D. *, p < 0.05; **, p < 0.01; and ***, p < 0.001 using analysis of variance analysis.