FIGURE 2.

Follistatin-like domains are required for process-inducing activity. Schematic representation of the different TM-agrin constructs used to localize the region responsible for the formation of the filopodia-like processes (left). The different domains within the TM-agrin protein are specified on the bottom. The nine follistatin-like domains are numbered according to their position starting from the N terminus. Quantification of the transfected cells extending processes is shown on the right. Note that deletion of most of the extracellular part of TM-agrin has no influence on process formation. However, deletion of follistatin-like domains 7 and 8 (TM-agrin-FD6 construct) abolished process-inducing activity. The bars represent the mean ± S.E. with n = 3.