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Circular dichroism and fluorescence emission spectra of ALAS and the Ser-254 variants. Spectra of wild-type ALAS (–), S254A (···) and S254T (- - -). A and B, ligand-free holoenzymes; C, in the presence of 100 μm succinyl-CoA; D, upon excitation of the cofactor at 331 nm.
