TABLE 1.

Summary of steady-state kinetic parameters

Enzyme	KmGly	KmSCoA	kcat	kcat/KmGly	kcat/KmSCoA
	mm	μm	s−1	mm−1·s−1	μm−1 ·s−1
Wild type	25 ± 4	1.3 ± 0.9	0.14 ± 0.02	5.6 × 10−3	1.1 × 10−1
S254A	18 ± 2	32 ± 7	0.27 ± 0.01	1.5 × 10−2	8.4 × 10−3
S254T	27 ± 3	1.2 ± 0.3	0.050 ± 0.004	1.9 × 10−3	4.2 × 10−2