FIGURE 4.

FheCL1 inhibits TRIF-dependent production of inflammatory mediators. A, peritoneal macrophages isolated 2 h after a single 50 μl intraperitoneal injection of either FheCL1 (1 mg/ml) or PBS were stimulated with either LPS (1 μg/ml) or poly(I·C) (25 μg/ml). B, cell supernatants were then analyzed by ELISA, and cell lysates were examined by RT-PCR for levels of mRNA expression of cytokines. C, ELISA was used to measure the quantity of IL-12 in culture media or macrophages stimulated in vitro with the TLR9 ligand CpG (1 μm), the synthetic TLR2 ligand Pam₃Cys-Ser-(Lys)₄(10 ng/ml) and the TLR5 ligand flagellin (10 ng/ml). Indicated values for ELISA are the means + S.E. of triplicates and are representative of three individual experiments.