TABLE 2.

Affinity between BclxL and a fluorescein-labeled Bid BH3 peptide in the presence of p53DBD

Buffer conditions were: 10 mm sodium phosphate, pH 7.2, 1 mm DTT at room temperature. 750 nm FL-Bid was used.

[p53DBD variant]	KD(BclxL-Bid)
	p53DBD wild-type	p53DBD R273H
μm	μm
0	0.97	0.97
2	0.59	1.10
5	0.51	1.49
30	0.36	0.45