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. Author manuscript; available in PMC: 2010 Feb 17.
Published in final edited form as: Nature. 2008 May 28;454(7200):126–130. doi: 10.1038/nature06992

Figure 1. TLS is a specific CBP/p300 HAT inhibitor.

Figure 1

a, CBP HAT activity measured by “pull down” HAT assay. WCE, whole cell extract; NE, nuclear extract; Cyto, cytoplasmic extract. b, Top, CBP HAT inhibitory activity revealed by gel filtration chromatography. MW, molecular weight. Bottom, Profile of TLS detected by Western blotting (WB). c, Representative silver-stained gels of pooled high and low MW fractions. d, TLS interacts with CBP, p300 and TIP60, but not p/CAF. e, The effect of CBP HAT activity by GST-TLS on histones or p53.