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. 2009 Nov 9;285(6):3608–3616. doi: 10.1074/jbc.M109.066431

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FIGURE 1. — Design of gene expression array study, and quality control of SAFB1/SAFB2 siRNA in MCF-7 cells. A, model of experimental setup of gene expression array study. V, vehicle. B, specific and efficient down-regulation of SAFB1 and SAFB2 by siRNA. MCF-7 cells were transiently transfected with NS siRNA, SAFB1 siRNA, SAFB2 siRNA, or SAFB1/SAFB2 siRNA. mRNA levels of SAFB1 and SAFB2 were measured by q-PCR (top panel), and protein expression by immunoblotting (bottom panel) using antibodies as indicated.