FIGURE 2.

Confirmation of SAFB binding to candidate genes identified in ChIP-chip study. A, confirmation of SAFB binding to control genes hsp27/HSPB1 and pS2/TIFF1. Top panel, graphical presentation of SAFB1-binding peaks in hsp27/HSPB1 and pS2/TIFF1 genes, as identified in the ChIP-chip experiment. The black line around 1 represent the average binding (signal) across the entire array. The gene structure and the mammalian conservation of sequence are shown. Bottom panel, ChIP was performed using pan-SAFB antibodies and triplicate biological samples (primer sequences are provided in supplemental Table S1). B, same as in A, but with FRAT1 and OPG genes. ChIP assay, using SAFB1-specific antibodies, is representative of three independent experiments.