TABLE 1.

Changes in the levels of plasma metabolites and insulin

At the age of 5–6 weeks, PFKFB3^+/− mice and wild-type littermates were fed an HFD or LFD for 12 weeks. After the feeding regimen, mice were fasted for 4 h before collection of plasma samples. Data are means ± S.E., n = 6.

	Wild-type	PFKFB3+/−
Glucose (mg/dl)
LFD	156 ± 16	202 ± 11a
HFD	238 ± 6b	308 ± 8bc
Insulin (ng/ml)
LFD	2.1 ± 0.3	3.2 ± 1.3
HFD	5.3 ± 1.6d	8.1 ± 0.8ab
Leptin (ng/ml)
LFD	4.2 ± 1.3	2.0 ± 0.7
HFD	65.0 ± 6.6b	43.8 ± 3.7ab
Free fatty acids (mm)
LFD	0.1 ± 0	0.2 ± 0a
HFD	0.2 ± 0.1d	0.3 ± 0d
Triglycerides (mg/dl)
LFD	34 ± 1	40 ± 3a
HFD	48 ± 4d	51 ± 2d

^a p < 0.05 PFKFB3^+/− vs. wild-type on the same diet.

^b p < 0.01 HFD vs. LFD for the same genotype.

^c p < 0.01 PFKFB3^+/− vs. wild-type on the same diet.

^d p < 0.05 HFD vs. LFD for the same genotype.