FIGURE 1.

AIP1 inhibits LPS-induced NF-κB and MAPK activation. Effects of AIP1 in LPS-induced NF-κB and MAPK activation in MLEC. WT and AIP1-KO MLEC (1 × 10⁶) were treated with LPS (1 ng/ml) for the indicated times. Phospho- and total IκBβ and p38 were determined by immunoblotting with the respective antibodies (a). The quantification of total IκBβ, and the ratios of p-IκBβ/IκB and p-p38/p38 are presented in b–d. Nuclear extracts from WT and AIP1-KO EC (0, 30, and 60 min) were also prepared for EMSA using a NF-κB probe (e). f and g, effects of AIP1 in TLR4-mediated NF-κB and AP-1 activation. 293T cells (1 × 10⁶) were transfected with the indicated reporter gene (0.l μg) and expression plasmids (0.5 μg). Data are the means ± S.D. from three independent experiments. Rel. Lucif. Act., relative luciferase activity.