FIGURE 1.

Current measurement of cytoplasmic domain deletion KcsA mutants. Single channel currents of mutant channels reconstituted into an artificial planar lipid bilayer were recorded in a symmetrical solution containing 200 mm KCl and 10 mm Tris-MES, pH 4.0. A, representative current records of KcsA E71A (control), KcsAΔ125–160, KcsAΔ135–160, and KcsAΔ145–160 at 60 mV are shown. B, I-V relationships of cytoplasmic domain deletion mutants are shown. ●, KcsA E71A; ■, KcsAΔ125–160; ▴, KcsAΔ135–160; ♦, KcsAΔ145–160.