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Current measurement of TMR-labeled KcsA mutants. Single channel currents of mutant channels reconstituted into an artificial planar lipid bilayer were recorded in a symmetrical solution containing 200 mm KCl and 10 mm Tris-MES, pH 4.0. A, representative current records of mutant channels (G53C and A132C) are shown. B, I-V relationships of TMR-labeled KcsA mutants are shown.
