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Emission spectra of TMR-A132C and TMR-G53C. We measured fluorescence intensities of the TMR-labeled KcsA mutants A132C (A) and G53C (B) after reconstituting them into liposomes. The excitation wave length was 532 nm. The data were normalized at 570 nm and pH 4. C, spectra of A132C and G53C are superimposed.
