TABLE 1.
Channel properties of cytoplasmic domain deletion KcsA mutants
Chord conductances and open probabilities of full-length KcsA(E71A) and cytoplasmic domain deletion KcsA mutants were measured at 100 mV in a symmetrical solution containing 200 mm KCl and 10 mm Tris-MES, pH 4.0 (mean ± S.D., n = 3–9).
Chord conductances | Open probability | |
---|---|---|
pS | ||
E71A (control) | 92 ± 8 | 0.91 ± 0.06 |
KcsA Δ125–160 | 50 ± 11 | 0.68 ± 0.13 |
KcsA Δ135–160 | 43–90 | 0.84 ± 0.10 |
KcsA Δ145–160 | 144 ± 18 | 0.97 ± 0.04 |