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. 2009 Dec 9;285(6):3850–3856. doi: 10.1074/jbc.M109.070839

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FIGURE 6. — Sus1 binds to Sac3 and Sgf11 in a mutually exclusive manner. GST-Sac3 753–805 (containing the Sus1B-binding site) and GST-Sac3 723–805 (containing the Sus1A and Sus1B-binding sites) were co-expressed with Cdc31 and immobilized on glutathione Sepharose 4B resin from clarified lysates. After resin washing, purified Sgf11-Sus1 complex was added and incubated. The unbound fraction and washed resin was analyzed by SDS-PAGE. Sgf11 is not observed in the bound material whereas Sus1 is present indicating the inability of Sus1 to bind Sac3 and Sgf11 simultaneously.