FIGURE 5.

Two-dimensional ¹H-¹H tr-NOESY spectra of Pa4 in LPS. A, the finger print region of the tr-NOESY spectra of Pa4 showing sequential and some medium range NOEs involving C^αH and HN resonances. C and D, long range NOEs between the amide proton (C) and aromatic ring protons (D) of residue Phe¹⁵ with residues Ile⁹, Ile¹⁹, and Ala²¹. B, spin system identification of residue Ile⁹ of Pa4 in ¹H-¹H two-dimensional TOCSY spectrum. The tr-NOESY spectra of Pa4 were acquired in the presence of 40 μm LPS. tr-NOESY experiments were carried out at 600 MHz and 288 K, with a mixing time of 150 ms.