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. 2009 Dec 1;285(6):3939–3948. doi: 10.1074/jbc.M109.044305

TABLE 1.

EMSA probe and competitor oligonucleotides

For each probe and competitor, the complementary sequences are shown. Oligonucleotide positions are numbered with respect to the transcription initiation site. NRF-2 core recognition sequences are in boldface type, and mutated nucleotides are underlined; gt nucleotides were artificially added to each oligonucleotide.

Promoter Sequence Position
RCOX4 5′-gtCTTGCTCTTCCGGTGCGGGACCCGCTCTTCCGGTCGCGA-3′ See Ref. 18
5′-gtTCGCGACCGGAAGAGCGGGTCCCGCACCGGAAGAGCAAG-3′
mTERF (−48/−29 probe) 5′-gtGCGACCCGAGGCCGGAAGTTAGTCTTTTCCACTTCCGCTCCTCCTAGCC-3′ −61/−13
5′-gtGGCTAGGAGGAGCGGAAGTGGAAAAGACTAACTTCCGGCCTCGGGTCGC-3′
POLRMT (−61/−45 probe) 5′-gtTCCTGGGAGTCTACTTCCGGCTGGGGTTTCCCTTCGCAGCCTCCGTCG-3′ −75/−28
5′-gtCGACGGAGGCTGCGAAGGGAAACCCCAGCCGGAAGTAGACTCCCAGGA-3′
POLRMT (mut-61 competitor) 5′-gtTCCTGGGAGTCTACTAGAGGCTGGGGTTTCCCTTCGCAGCCTCCGTCG-3′ −75/−28
5′-gtCGACGGAGGCTGCGAAGGGAAACCCCAGCCTCTAGTAGACTCCCAGGA-3′
POLRMT (mut-45 competitor) 5′-gtTCCTGGGAGTCTACTTCCGGCTGGGGTTTCAGATCGCAGCCTCCGTCG-3′ −75/−28
5′-gtCGACGGAGGCTGCGATCTGAAACCCCAGCCGGAAGTAGACTCCCAGGA-3′
TWINKLE (−45 probe) 5′-gtACTAAACCTCGAGGCTTCCGGTTCCGGGACGACCGCTCCC-3′ −60/−21
5′-gtGGGAGCGGTCGTCCCGGAACCGGAAGCCTCGAGGTTTAGT-3′
mtSSB (−11 probe) 5′-gtTTTGCGTTCCCTGTGCGCCGGAAGTGATCCCCTGCGTGGC-3′ −30/+10
5′-gtGCCACGCAGGGGATCACTTCCGGCGCACAGGGAACGCAAA-3′
PolA (−22 probe) 5′-gtCCTAGCTGGGTGCAGACGGGAAGTTGCGGCTGCCAGCGAA-3′ −40/−1
5′-gtTTCGCTGGCAGCCGCAACTTCCCGTCTGCACCCAGCTAGG-3′
PolB (−61 probe) 5′-gtCCGGAACGCAGACATGCGCTTCCGGGGTGGGGCCTGCCGC-3′ −80/−41
5′-gtGCGGCAGGCCCCACCCCGGAAGCGCATGTCTGCGTTCCGG-3′
PolB (−255 probe) 5′-gtTGCAAAACAGTGGCAGAAGGAAGTGACTCAAGTGGCTACA-3′ −273/−234
5′-gtTGTAGCCACTTGAGTCACTTCCTTCTGCCACTGTTTTGCA-3′