FIGURE 8.

In vivo analyses showing ω-hydroxy products. Acyl-CoAs were extracted from the wild-type stigma (upper panel) and the CYP86A22-RNAi type VI stigma (lower panel). The ω-hydroxy acyl-CoA products were directly analyzed by LC/MS/MS. The ω-hydroxy 18:1-CoA was monitored by the precursor-product ion pair of m/z 1048.35 and 541.36, and ω-hydroxy 18:2-CoA was monitored by the precursor-product ion pair of m/z 1046.33 and 539.34. The ion intensity was measured as counts per second (cps). The arrows indicate the corresponding ω-hydroxy acyl-CoAs disappearing in the CYP86A22-RNAi stigma.