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. 2009 Dec 3;285(6):3997–4005. doi: 10.1074/jbc.M109.034421

FIGURE 7.

FIGURE 7.

Effects of TXNIP knockdown on Trx2-ASK1 interaction and ASK1 phosphorylation/activation. A, demonstration of specific TXNIP knockdown by siRNA in INS-1 cells. Cells were transfected with TXNIP siRNA (siTXNIP) or scrambled (scram) oligonucleotides as described under “Experimental Procedures” and analyzed 48 h later by immunoblotting for TXNIP as well as for Trx1 and Trx2 protein expression. B and C, Trx2-ASK1 interaction and ASK1 phosphorylation/activation, respectively, in response to TXNIP knockdown as assessed by co-immunoprecipitation (IP). Error bars represent means ± S.E. of at least three independent experiments. p-ASK1, phosphorylated ASK1; t-ASK1, total ASK1.