FIGURE 2.

Adenovirus-c-Jun stimulates SMC proliferation. A, expression of c-Jun, PCNA, and MMP-2 in rat SMCs after transduction with adenovirus-LacZ or adenovirus c-Jun. Twenty-four hours after transduction, total cell extracts were prepared in radioimmune precipitation assay buffer prior to Western blot analysis with appropriate antibodies. B, rat SMCs in 96-well plates (1000 cells seeded per well) were transduced with adenovirus and after 24 h incubated in serum-free media for a further 24 h. The cells were then incubated in medium containing 5% FBS for up to 2 days subsequently. Days 2, 3, and 4 refer to the period since adenovirus transduction. The data are representative of at least two experiments performed in triplicate.